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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1983-4-15
|
| pubmed:abstractText |
A case of human sympathetic ophthalmia, enucleated after surgical trauma, was studied by means of hybridoma-derived monoclonal antibodies, histochemistry, and transmission electron microscopy. The choroidal infiltrate was composed predominantly of T-lymphocytes of the suppressor/cytotoxic subset (OKT8+); only 5% of the cells were immunoglobulin-producing B-lymphocytes (kappa or lambda light chain positive), thereby explaining the well-known paucity of plasma cells in the infiltrate. The epithelioid cells and phagocytic histiocytes in the choroid were la+ and OKM1+, antigenic determinants specific for bone marrow-derived monocytes, and their cytoplasms exhibited histochemical reactivity for alpha-1-antichymotrypsin and lysozyme. Ultrastructurally, the choroidal epithelioid cells contained single melanin granules in the cytoplasm, but these were membrane-bound and frequently associated with lysosomal material, features militating against these cells being transformed choroidal melanocytes. By means of immunologic and ultrastructural analysis, the Dalen-Fuchs nodules were found to be composed of a mixture of histiocytes (la+ and OKM1+) and depigmented retinal pigment epithelial cells (la- and OKM1-); the latter cells focally formed desmosomes and displayed inclusions of lipofuscin. Scattered within the Dalen-Fuchs nodules were small numbers of T-lymphocytes of the suppressor/cytotoxic subset. We have concluded that the uveitis and retinal pigment epithelial changes are mediated by a T-cell, delayed hypersensitivity pathogenetic mechanism (cell-mediated immunity), possibly directed at surface membrane antigens that may be shared by photoreceptors, retinal pigment epithelial cells, and choroidal melanocytes.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0161-6420
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
90
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
76-95
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:6338439-Animals,
pubmed-meshheading:6338439-Antibodies, Monoclonal,
pubmed-meshheading:6338439-B-Lymphocytes,
pubmed-meshheading:6338439-Cell Count,
pubmed-meshheading:6338439-Choroid,
pubmed-meshheading:6338439-Female,
pubmed-meshheading:6338439-Fluorescent Antibody Technique,
pubmed-meshheading:6338439-Humans,
pubmed-meshheading:6338439-Hybridomas,
pubmed-meshheading:6338439-Immunoenzyme Techniques,
pubmed-meshheading:6338439-Leukocyte Count,
pubmed-meshheading:6338439-Macrophages,
pubmed-meshheading:6338439-Mice,
pubmed-meshheading:6338439-Microscopy, Electron,
pubmed-meshheading:6338439-Middle Aged,
pubmed-meshheading:6338439-Ophthalmia, Sympathetic,
pubmed-meshheading:6338439-Pigment Epithelium of Eye,
pubmed-meshheading:6338439-Retina,
pubmed-meshheading:6338439-T-Lymphocytes
|
| pubmed:year |
1983
|
| pubmed:articleTitle |
Human sympathetic ophthalmia. An analysis of the inflammatory infiltrate by hybridoma-monoclonal antibodies, immunochemistry, and correlative electron microscopy.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Case Reports,
Research Support, Non-U.S. Gov't
|