Linked Life Data

6332614

Source:http://linkedlifedata.com/resource/pubmed/id/6332614

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
74
pubmed:dateCreated
1984-10-25
pubmed:abstractText
Six antigenic systems on melanoma cell surfaces have been defined by monoclonal antibodies. The disialoganglioside GD3, detected by mAb R-24, was found to be strongly expressed on most melanoma cell surfaces, but weakly on normal melanocytes and some astrocytomas as determined by serological methods. Epithelial cell types, fibroblasts and cells of hematopoietic origin did not react. Frozen sections of primary and metastatic malignant melanoma stained positive in 60/62 cases by immunofluorescence and immunoperoxidase. In vitro studies with seven antibodies to seven melanoma cell surface antigens revealed blocking of melanoma cell growth by monoclonal anti-GD3-antibody R-24 only. GD3-expression varies greatly in human malignant melanoma cells. Six melanoma cell lines, expressing high levels of GD3 on the cell surface, as determined by quantitative absorption studies, showed growth inhibition in the presence of R-24 antibody. Four melanoma cell lines, however, with low GD3-expression and seven GD3-negative non-melanoma cells types remained unaffected by mAbR-24. In addition, this monoclonal anti-GD3-antibody efficiently blocked T-cell cytotoxicity for autologous malignant melanoma cells in culture. Cytotoxicity of autologous cloned T-lymphocytes, cytotoxic for the autologous melanoma cell line and crossreactive with a hypernephroma line was specifically blocked by mAbR-24 on the level of the GD3-expressing target. In an ongoing phase I clinical study, the toxicity of mAbR-24 was evaluated. So far two patients tolerated antibody infusions without adverse reactions.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0301-0457
pubmed:author
pubmed:issnType
Print
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
14-8
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Melanoma antibodies: specificity and interaction with melanoma and cytotoxic T-cells.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't