Linked Life Data

6330931

Source:http://linkedlifedata.com/resource/pubmed/id/6330931

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1984-8-3
pubmed:abstractText
Mouse C1q, a subcomponent of the first component of complement, was purified from mouse EDTA plasma by a combination of precipitation with polyethyleneglycol, affinity chromatography on IgG-Sepharose, ion exchange chromatography and molecular sieving. Mouse C1q was compatible with human C1q in the sense that it shows C1 hemolytic activity by the combination with human C1-r and C1-s. The molecular weight of mouse C1q as estimated by SDS-PAGE was approximately 410,000 and almost the same as that of human C1q. After treatment of mouse C1q with 4 M urea, three distinct subunits were obtained on SDS-PAGE in non-reducing condition. Moreover it was shown that there are small, yet definite differences in the molecular weights of the subunits between the two mouse strains. Three subunits of the C1q from C3H and ICR showed apparent molecular weights of 62,000, 58,000 and 53,000 but those of DDI showed 62,000, 55,000 and 51,000. These results suggested the existence of allotype of mouse C1q, and indeed, alloantisera made by injecting the C1q of ICR to DDI reacted in Ouchterlony double diffusion test with plasma of ICR, C3H, AKR and BALB/c, but not with DDD, DKI, C57BL/10 and C57BL/6.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0040-8727
pubmed:author
pubmed:issnType
Print
pubmed:volume
142
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
351-61
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Isolation, molecular properties and allotype of mouse C1q.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't