Linked Life Data

6329229

Source:http://linkedlifedata.com/resource/pubmed/id/6329229

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1984-6-22
pubmed:abstractText
We have investigated the action of a protein synthesis inhibitor on the ability of angiotensin II (AII) to stimulate steroid synthesis. Isolated bovine adrenal glomerulosa cells were incubated in the presence and absence of angiotensin and cycloheximide, and the effects of the inhibitor on six cellular processes were measured. Cycloheximide at 7 and 28 microM inhibited the ability of the hormone to stimulate aldosterone synthesis. These concentrations of cycloheximide blocked protein synthesis by 72 and 79% respectively. Cycloheximide did not block receptor binding of angiotensin, the effect of angiotensin on [32P]phosphate incorporation into phosphatidylinositol, nor the ability of the hormone to alter 45Ca2+ fluxes. Mitochondrial conversion of cholesterol to pregnenolone is thought to be the rate-determining step in corticosteroid synthesis. Mitochondria isolated from cells treated with angiotensin made pregnenolone at a higher rate than control mitochondria. Cycloheximide blocked this effect when it was present in the cell incubation medium with angiotensin. Cycloheximide added directly to mitochondria had no effect on pregnenolone synthesis. Cycloheximide also blocked AII stimulation of pregnenolone synthesis in intact cells. We propose that protein synthesis is required for angiotensin to exert its stimulatory effects at one particular locus: activation of mitochondrial pregnenolone synthesis. Protein synthesis is not required for other angiotensin-stimulated processes in bovine adrenal glomerulosa cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-2952
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1519-24
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Identification of the cycloheximide-sensitive site in angiotensin-stimulated aldosterone synthesis.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, Non-P.H.S.