Linked Life Data

6323715

Source:http://linkedlifedata.com/resource/pubmed/id/6323715

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-5-16
pubmed:abstractText
Electrical properties of isolated frog primary afferent neurons were examined by suction pipette technique, which combines internal perfusion with current or voltage clamp using a switching circuit with a single electrode. When K+ in the external and internal solutions was totally replaced with Cs+, extremely prolonged Ca spikes, lasting for 5 to 10 sec, and Na spikes, having a short plateau phase of 10 to 15 msec, were observed in Na+-free and Ca2+-free solutions, respectively. Under voltage clamp, Ca2+ current (ICa) appeared at around -30 mV and maximum peak current was elicited at about 0 mV. With increasing test pulses to the positive side, ICa became smaller and flattened but did not reverse. Increases of [Ca]o induced a hyperbolic increase of ICa and also shifted its I-V curve along the voltage axis to the more positive direction. Internal perfusion of F- blocked ICa time-dependently. The Ca channel was permeable to foreign divalent cations in the sequence of ICa greater than IBa greater than ISr much greater than IMn greater than IZn. Organic Ca-blockers equally depressed the divalent cation currents dose- and time-dependently without shifting the I-V relationships, while inorganic blockers suppressed these currents dose-dependently and the inhibition appeared much stronger in the order of IBa = ISr greater than ICa greater than IMn = IZn.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0022-2631
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19-28
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Separation of ionic currents in the somatic membrane of frog sensory neurons.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't