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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1983-12-21
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pubmed:databankReference |
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pubmed:abstractText |
Fractionation of HeLa cell extracts reveals the presence of a promoter-specific transcription factor, Sp 1, which activates a class of promoters that includes the SV40 early promoter but not several others that have been tested. We analyzed SV40 early-promoter deletion mutants and determined that transcriptional activation by Sp 1 requires sequences within tandem 21 bp repeats located 70-110 bp upstream of the transcription initiation sites. In a DNAase footprinting assay, Sp 1 protected sequences in this same 21 bp repeat region, thus indicating the presence of a specific site for Sp 1 binding. During purification of Sp 1, there was a correlation between transcription-stimulatory activity and promoter-binding activity. These results suggest that direct binding of Sp 1 to sequences in the upstream promoter element is the mechanism by which this factor activates transcription by RNA polymerase II at the SV40 early promoter.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
79-87
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6313230-Binding Sites,
pubmed-meshheading:6313230-DNA, Viral,
pubmed-meshheading:6313230-Genes, Viral,
pubmed-meshheading:6313230-HeLa Cells,
pubmed-meshheading:6313230-Humans,
pubmed-meshheading:6313230-Mutation,
pubmed-meshheading:6313230-Operon,
pubmed-meshheading:6313230-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:6313230-Simian virus 40,
pubmed-meshheading:6313230-Transcription, Genetic,
pubmed-meshheading:6313230-Transcription Factors
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pubmed:year |
1983
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pubmed:articleTitle |
The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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