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rdf:type |
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|
lifeskim:mentions |
|
|
pubmed:issue |
3
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|
pubmed:dateCreated |
1983-8-11
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pubmed:abstractText |
A simple and rapid assay method is described for determining the specific radioactivity of the gamma-phosphate group of 32P-labelled ATP. Labelled ATP is incubated with cyclic AMP, cyclic AMP-dependent protein kinase and histone H2A under conditions leading to maximum phosphorylation of the histone. The specific radioactivity of the gamma-phosphate group of the 32P-labelled ATP is calculated from the total amount of [32P]Pi incorporated into a standard amount of histone. This assay method uses inexpensive commercially available materials, and it yields an accurate specific radioactivity with as little as 0.25 nmol of 32P-labelled ATP.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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|
pubmed:month |
Mar
|
|
pubmed:issn |
0264-6021
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
15
|
|
pubmed:volume |
210
|
|
pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
717-20
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
|
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pubmed:year |
1983
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pubmed:articleTitle |
A simple assay method for determination of the specific radioactivity of the gamma-phosphate group of 32P-labelled ATP.
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|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
