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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1983-6-17
|
| pubmed:abstractText |
Tetracycline-resistance (TCr) plasmid pTP-5 (4.46 kb) from Staphylococcus aureus was cleaved with HindIII into three fragments: A (2.35 kb), B (1.57 kb) and C (0.54 kb). A deletion plasmid (3.92 kb) lacking HindIII-C fragment was obtained, and was designated pNS1. This plasmid retained the TCr phenotype and the ability to replicate autonomously in Bacillus subtilis. A restriction endonuclease cleavage map of pNS1 was constructed. Attempts to construct smaller plasmids by digesting pNS1 with BAL31 nuclease led to production of a set of deletion derivatives whose molecular sizes range from 3.75 to 2.77 kb. Through analyses of these derivatives, the regions essential for autonomous replication and expression of TCr were deduced.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0378-1119
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
21
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
105-10
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6301940-Bacillus subtilis,
pubmed-meshheading:6301940-Chromosome Deletion,
pubmed-meshheading:6301940-Chromosomes, Bacterial,
pubmed-meshheading:6301940-DNA Restriction Enzymes,
pubmed-meshheading:6301940-Drug Resistance, Microbial,
pubmed-meshheading:6301940-Phenotype,
pubmed-meshheading:6301940-Plasmids,
pubmed-meshheading:6301940-Staphylococcus aureus,
pubmed-meshheading:6301940-Tetracycline
|
| pubmed:articleTitle |
Construction and propagation of deletion derivatives of staphylococcal tetracycline-resistance plasmid pTP-5 in Bacillus subtilis.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|