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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1983-5-27
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pubmed:abstractText |
A variant of the ELISA technique, involving a monoclonal anti-gp51 antibody yields a highly sensitive method for the detection of bovine leukaemia virus (BLV) antibodies. The gp51 antigen-coated microtitre plates are obtained by incubation of plastic-adsorbed monoclonal antibodies with a non-purified mixture of BLV antigens. Sera to be tested are incubated in the wells of the gp51-coated plates and bound antibodies are revealed by an enzyme-linked antibovine immunoglobulin reagent. This test is as sensitive as liquid phase radioimmunoassay using the same gp51 antigen and thus appears as a highly sensitive, practical, rapid and cheap method for the detection of BLV antibodies.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0166-0934
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
19-29
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6300156-Animals,
pubmed-meshheading:6300156-Antibodies, Monoclonal,
pubmed-meshheading:6300156-Antibodies, Viral,
pubmed-meshheading:6300156-Cattle,
pubmed-meshheading:6300156-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:6300156-Immunoenzyme Techniques,
pubmed-meshheading:6300156-Leukemia Virus, Bovine,
pubmed-meshheading:6300156-Radioimmunoassay,
pubmed-meshheading:6300156-Retroviridae,
pubmed-meshheading:6300156-Temperature,
pubmed-meshheading:6300156-Time Factors,
pubmed-meshheading:6300156-Viral Envelope Proteins,
pubmed-meshheading:6300156-Viral Proteins
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pubmed:year |
1983
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pubmed:articleTitle |
Use of monoclonal antibody in an ELISA test for the detection of antibodies to bovine leukaemia virus.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|