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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1983-3-11
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pubmed:abstractText |
Highly purified sarcolemmal membranes were prepared from pig heart homogenates by differential and density gradient centrifugations. The membrane fragments exhibit ATP-dependent Ca2+-transport and Na+/Ca2+-exchange activities. ATP-dependent Ca2+-transport (K0.5Ca2+ = 0.3 microM; Vmax = 4.6 nmol Ca2+.mg protein-1.min-1) is not stimulated by oxalate. Ca2+-uptake is also not supported by p-nitro-phenylphosphate. Preincubation of sarcolemma with MgATP, calmodulin and catalytic subunit of cyclic AMP-dependent protein kinase stimulates active Ca2+-transport 1.8-fold. The effects of calmodulin and catalytic subunit are potentiating rather than additive. A large portion of the Ca2+ additionally accumulated after prephosphorylation of membranes is exchangeable for Na+ via the Na+/Ca2+-exchange system.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
148
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
326-30
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:6295809-Adenosine Triphosphate,
pubmed-meshheading:6295809-Animals,
pubmed-meshheading:6295809-Biological Transport, Active,
pubmed-meshheading:6295809-Calcium,
pubmed-meshheading:6295809-Calcium-Binding Proteins,
pubmed-meshheading:6295809-Calcium-Transporting ATPases,
pubmed-meshheading:6295809-Calmodulin,
pubmed-meshheading:6295809-Cell Membrane,
pubmed-meshheading:6295809-Cyclic AMP,
pubmed-meshheading:6295809-Heart Ventricles,
pubmed-meshheading:6295809-Kinetics,
pubmed-meshheading:6295809-Myocardium,
pubmed-meshheading:6295809-Protein Kinases,
pubmed-meshheading:6295809-Sarcolemma,
pubmed-meshheading:6295809-Swine
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pubmed:year |
1982
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pubmed:articleTitle |
Potentiating effect of calmodulin and catalytic subunit of cyclic AMP-dependent protein kinase on ATP-dependent Ca2+-transport by cardiac sarcolemma.
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pubmed:publicationType |
Journal Article
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