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rdf:type |
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lifeskim:mentions |
umls-concept:C0017428,
umls-concept:C0035668,
umls-concept:C0178774,
umls-concept:C0220781,
umls-concept:C1444754,
umls-concept:C1533691,
umls-concept:C1623048,
umls-concept:C1706853,
umls-concept:C1879748,
umls-concept:C1883254,
umls-concept:C1948027
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pubmed:issue |
20
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pubmed:dateCreated |
1983-2-14
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pubmed:abstractText |
Synthesis of a small amount of 42S RNA in addition to the VSV specific mRNA species was observed in a coupled transcription-translation system containing ribonucleoprotein particles from L cell infected with vesicular stomatitis virus and nuclease-treated ribosomal extract obtained from uninfected HeLa cells. Analysis on a CsCl density gradient showed that the synthesized 42S RNA was associated with newly synthesized by protein as a nucleoprotein of bouyant density of 1.3 g/ml. The 42S RNA and the N protein present in the nucleoprotein were resistant to nuclease and protease, respectively. About 35% of the remaining 65% had a complementary polarity. The evidence presented here demonstrates that both the full length genomic and the complementary RNA are associated with N protein in the in vitro replication process. A template role for the complementary 42S RNA for replication of the genomic RNA is also suggested.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-167189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-176426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-183350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-193104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-204929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-206007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-214246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-218209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-223312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-228051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-4342247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-4355930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-4357053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-4357512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-4361452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-4369854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-6244552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-6257927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-6267779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-6284973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-6976837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294600-823012
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6341-51
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6294600-Animals,
pubmed-meshheading:6294600-Capsid,
pubmed-meshheading:6294600-Genes, Viral,
pubmed-meshheading:6294600-L Cells (Cell Line),
pubmed-meshheading:6294600-Mice,
pubmed-meshheading:6294600-Protein Biosynthesis,
pubmed-meshheading:6294600-RNA, Viral,
pubmed-meshheading:6294600-Ribonucleoproteins,
pubmed-meshheading:6294600-Transcription, Genetic,
pubmed-meshheading:6294600-Vesicular stomatitis Indiana virus,
pubmed-meshheading:6294600-Viral Proteins
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pubmed:year |
1982
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pubmed:articleTitle |
Synthesis in vitro of full length genomic RNA and assembly of the nucleocapsid of vesicular stomatitis virus in a coupled transcription-translation system.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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