Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1982-9-10
pubmed:abstractText
A method is described for the determination of 5'-nucleotidase activity in human erythrocytes and plasma. Using reversed-phase high-performance liquid chromatography; the product (uridine) was separated from the substrate (uridine-5'-monophosphate) in less than 4 min. The activity determined closely agreed with that determined by the conventional method, in which the inorganic phosphate released is measured. The present method eliminates the need for dialysis of enzyme solution prior to the assay, and offers several advantages over other assay methods, including high sensitivity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
239
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
717-21
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Determination of 5'-nucleotidase activity in human erythrocytes and plasma using high-performance liquid chromatography.
pubmed:publicationType
Journal Article