| pubmed-article:6280195 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6280195 | lifeskim:mentions | umls-concept:C0013671 | lld:lifeskim |
| pubmed-article:6280195 | lifeskim:mentions | umls-concept:C0013782 | lld:lifeskim |
| pubmed-article:6280195 | lifeskim:mentions | umls-concept:C1148479 | lld:lifeskim |
| pubmed-article:6280195 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:6280195 | lifeskim:mentions | umls-concept:C1179435 | lld:lifeskim |
| pubmed-article:6280195 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:6280195 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:6280195 | lifeskim:mentions | umls-concept:C1705248 | lld:lifeskim |
| pubmed-article:6280195 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:6280195 | lifeskim:mentions | umls-concept:C1548799 | lld:lifeskim |
| pubmed-article:6280195 | lifeskim:mentions | umls-concept:C1524073 | lld:lifeskim |
| pubmed-article:6280195 | lifeskim:mentions | umls-concept:C0449432 | lld:lifeskim |
| pubmed-article:6280195 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:6280195 | pubmed:dateCreated | 1982-6-21 | lld:pubmed |
| pubmed-article:6280195 | pubmed:abstractText | A monoclonal hybridoma cell line secreting antibody against the saxitoxin-binding component from the eel Electrophorus electricus has been isolated. The specificity of this monoclonal antibody was established by (i) its ability to immunoprecipitate bound [3H]saxitoxin from a detergent extract of electroplax membranes in a dose-dependent manner, (ii) the inability of unrelated monoclonal antibodies to immunoprecipitate the toxin-binding activity in a similar assay, and (iii) the ability of excess unlabeled tetrodotoxin to displace [3H]saxitoxin from the immunoprecipitated component. The antibody is of the subclass IgG1 and binds specifically to a polypeptide component of Mr approximately 250,000 on NaDodSO4/polyacrylamide gels. The antigenic determinant is associated with the same polypeptide component throughout the purification procedure, indicating that this component is not a result of artifactual aggregation or degradation during isolation. We conclude that the 250,000-dalton polypeptide is part of the saxitoxin binding/sodium channel protein in the native electroplax membrane. | lld:pubmed |
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| pubmed-article:6280195 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6280195 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6280195 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6280195 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6280195 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:6280195 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:6280195 | pubmed:author | pubmed-author:RafteryM AMA | lld:pubmed |
| pubmed-article:6280195 | pubmed:author | pubmed-author:MooreH PHP | lld:pubmed |
| pubmed-article:6280195 | pubmed:author | pubmed-author:BrockesJ PJP | lld:pubmed |
| pubmed-article:6280195 | pubmed:author | pubmed-author:FritzL CLC | lld:pubmed |
| pubmed-article:6280195 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6280195 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:6280195 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6280195 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6280195 | pubmed:pagination | 1673-7 | lld:pubmed |
| pubmed-article:6280195 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6280195 | pubmed:meshHeading | pubmed-meshheading:6280195-... | lld:pubmed |
| pubmed-article:6280195 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:6280195 | pubmed:articleTitle | Isolation and characterization of a monoclonal antibody against the saxitoxin-binding component from the electric organ of the eel Electrophorus electricus. | lld:pubmed |
| pubmed-article:6280195 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6280195 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:6280195 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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