Linked Life Data

6280195

Source:http://linkedlifedata.com/resource/pubmed/id/6280195

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1982-6-21
pubmed:abstractText
A monoclonal hybridoma cell line secreting antibody against the saxitoxin-binding component from the eel Electrophorus electricus has been isolated. The specificity of this monoclonal antibody was established by (i) its ability to immunoprecipitate bound [3H]saxitoxin from a detergent extract of electroplax membranes in a dose-dependent manner, (ii) the inability of unrelated monoclonal antibodies to immunoprecipitate the toxin-binding activity in a similar assay, and (iii) the ability of excess unlabeled tetrodotoxin to displace [3H]saxitoxin from the immunoprecipitated component. The antibody is of the subclass IgG1 and binds specifically to a polypeptide component of Mr approximately 250,000 on NaDodSO4/polyacrylamide gels. The antigenic determinant is associated with the same polypeptide component throughout the purification procedure, indicating that this component is not a result of artifactual aggregation or degradation during isolation. We conclude that the 250,000-dalton polypeptide is part of the saxitoxin binding/sodium channel protein in the native electroplax membrane.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-275831, http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-351332, http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-4113792, http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-532965, http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-6246486, http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-6247957, http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-6256767, http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-6270687, http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-6928649, http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-7038023, http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-7362610, http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-825377, http://linkedlifedata.com/resource/pubmed/commentcorrection/6280195-978583
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1673-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Isolation and characterization of a monoclonal antibody against the saxitoxin-binding component from the electric organ of the eel Electrophorus electricus.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't