Linked Life Data

6273403

Source:http://linkedlifedata.com/resource/pubmed/id/6273403

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
1982-2-22
pubmed:abstractText
Protocatechuate dioxygenase has been isolated from Pseudomonas putida. This new species of protocatechuate dioxygenase has been characterized and compared with the enzyme from Pseudomonas aeruginosa. The enzyme reported here has visible absorption, circular dichroism, electron paramagnetic resonance, and Raman spectroscopic properties virtually identical to those for protocatechuate dioxygenase from P. aeruginosa. However, the molecular weight and iron:subunit stoichiometry differ. Protocatechuate dioxygenase from P. putida has a molecular weight of 200,000 and contains 4 alpha subunits of 23,000 daltons, 4 beta subunits of 26,500 daltons, and 4 ferric irons suggesting that the enzyme is a tetramer of (alpha beta Fe+3) catalytic units. Protocatechuate dioxygenase from P. aeruginosa has been reported to have a Mr = 700,000, consisting of 16 alpha subunits of 22,500 daltons, 16 beta subunits of 25,000 daltons, and 8 ferric irons (Yoshida, R., Hori, K., Fujiwara, M., Saeki, Y., Kagamiyama, H., and Nozaki, W. (1976) Biochemistry 15, 4048-4053). This enzyme is thought to be an octamer of (alpha 2 beta 2 Fe+3) catalytic units, although reconstitution with extra iron will somewhat increase its activity. Using stopped flow techniques, we have shown that essentially all of the iron in the P. putida enzyme is catalytically active. This suggests that the minimal catalytic unit of all non-heme iron intradiol dioxygenases is an (alpha beta Fe+3) structure.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
256
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12673-80
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Purification and properties of protocatechuate 3,4-dioxygenase from Pseudomonas putida. A new iron to subunit stoichiometry.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.