Linked Life Data

6270259

Source:http://linkedlifedata.com/resource/pubmed/id/6270259

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 2
pubmed:dateCreated
1981-12-21
pubmed:abstractText
In order to understand the mechanism of congenital cytomegalovirus (CMV) infection, we studied the effect of murine cytomegalovirus (MCMV) on murine ectoplacental cone (EPC) cells in vitro. Cytopathic effects (c.p.e.) were seen in many MCMV-infected EPC cell cultures 5 to 7 days after exposure to MCMV. The infected cells showed intranuclear inclusions characteristic of CMV infection when stained with May-Grunwald-Giemsa. Culture fluids collected from MCMV-infected EPC cells after 4 or more days of culture caused c.p.e. when co-cultivated with mouse embryo fibroblasts (MEF). Employment of the anti-complement immunofluorescence (ACIF) test detected MCMV-specific antigens, in situ hybridization localized MCMV DNA and electron microscopy detected the presence of the viral particles in the MCMV-infected EPC cells. Thus, exposure of EPC cells to MCMV in vitro resulted in a productive infection.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
415-20
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Infection of mouse ectoplacental cone cells with murine cytomegalovirus.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.