Linked Life Data

6261879

Source:http://linkedlifedata.com/resource/pubmed/id/6261879

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-7-20
pubmed:abstractText
We have previously shown that certain commercially available lots of alpha-bungarotoxin block transmission in ciliary and choroid neurons of both pigeon and chicken ciliary ganglia at a concentration of 10 microgram/ml (1.2 microM). The blockade is antagonized by pre-incubation with 100 microM tubocurarine. Further evidence that this blockade is produced by a postsynaptic action, as one would expect of an alpha-neurotoxin, are our findings that: (a) exposure to the toxin prevents the depolarization of ganglion cells normally seen in response to the cholinergic agonist, carbachol; and (b) the blocking activity of the toxin is removed by treatment with membranes purified from Torpedo electric organ containing an excess of alpha-neurotoxin binding sites. A high affinity binding site for [125I]alpha-bungarotoxin was characterized in the chicken ciliary ganglion. However, since it is labelled equally well by lots of alpha-bungarotoxin which block transmission and those that do not, this site does not appear to be involved in the blockade of transmission. alpha-Cobratoxin (from Naja naja siamensis), the alpha-neurotoxin L.s. III (from Laticauda semifasciata) and certain lots of alpha-bungarotoxin produce a partial blockade of transmission in ciliary neurons of the pigeon ciliary ganglion at a concentration of 10 microgram/ml (1.2 microM), but have no effect on transmission in choroid neurons. Two other alpha-neurotoxins from Laticauda semifasciata, erabutoxin a and erabutoxin b, have no effect on transmission in either cell population at this concentration. None of the alpha-neurotoxins tested had any effect on transmission in either the rat superior cervical ganglion or the rat pelvic ganglion at concentrations up to 100 microgram/ml (12 microM). Collagenase treatment of these ganglia, in an attempt to increase access of the toxins to ganglion cells, did not alter these negative results. beta-Bungarotoxin (0.5 microgram/ml, 0.02 microM) produces a complex blockade of transmission in both avian ciliary ganglia and rat superior cervical ganglia. Unlike the action of alpha-bungarotoxin, the blockade of ciliary ganglion transmission by beta-bungarotoxin is irreversible and is not prevented by pretreatment with tubocurarine.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
211
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-26
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
The effects of alpha- and beta-neurotoxins from the venoms of various snakes on transmission in autonomic ganglia.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.