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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
14
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pubmed:dateCreated |
1980-11-24
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pubmed:abstractText |
2-Stearoyl spin-labeled phosphatidylcholine (PC*) has been introduced into the phosphatidylcholine exchange protein from bovine liver and its electron spin resonance (ESR) spectrum determined. The spin-labeled group in the PC*- exchange protein complex was strongly immobilized. Addition of sodium deoxycholate micelles released PC* from its binding site, producing a mobile signal. This was also observed when micelles of lysophosphatidylcholine and vesicles of phosphatidic acid were added, indicating that the exchange protein can insert its endogenous PC* into interfaces devoid of phosphatidylcholine. ESR spectroscopy was used to measure transfer of PC* from spin-labeled "donor" vesicles to unlabeled "acceptor" vesicles as described by Machida & Ohnishi [Machida, K., & Ohnishi, S. (1978) Biochim. Biophys. Acta 507, 156-164]. The donor vesicles consisted of PC* and phosphatidic acid (75:25 mol%) and the acceptor vesicles of phosphatidylethanolamine and phosphatidic acid (81:19 mol%). Addition of exchange protein catalyzed a net transfer of PC* from donor to acceptor vesicles. This transfer proceeded until the acceptor vesicles contained approximately 2 mol% of PC*. A spontaneous transfer of PC* was not observed. As for the mode of action, it appears that the exchange protein, after insertion of its endogenous PC* into the acceptor, leaves the interface without a bound phospholipid molecule yet continues to shuttle PC* from donor to acceptor.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Androgen-Binding Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membranes, Artificial,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipid Transfer Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Spin Labels
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-2960
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3395-9
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pubmed:dateRevised |
2006-11-20
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pubmed:meshHeading |
pubmed-meshheading:6250569-Androgen-Binding Protein,
pubmed-meshheading:6250569-Animals,
pubmed-meshheading:6250569-Carrier Proteins,
pubmed-meshheading:6250569-Cattle,
pubmed-meshheading:6250569-Electron Spin Resonance Spectroscopy,
pubmed-meshheading:6250569-Kinetics,
pubmed-meshheading:6250569-Liver,
pubmed-meshheading:6250569-Membranes, Artificial,
pubmed-meshheading:6250569-Phosphatidylcholines,
pubmed-meshheading:6250569-Phospholipid Transfer Proteins,
pubmed-meshheading:6250569-Protein Binding,
pubmed-meshheading:6250569-Spin Labels
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pubmed:year |
1980
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pubmed:articleTitle |
Phosphatidylcholine exchange protein catalyzes the net transfer of phosphatidylcholine to model membranes.
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pubmed:publicationType |
Journal Article
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