Linked Life Data

6235813

Source:http://linkedlifedata.com/resource/pubmed/id/6235813

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
1984-9-4
pubmed:abstractText
The binding of [3H]spironolactone to kidney homogenates from adrenalectomized rats was studied by dextran-charcoal absorption methods. [3H]Spironolactone binds with high affinity and low capacity (KD = 12.9 +/- 0.6 nM; Bmax = 93.4 +/- 3.8 fmoles/mg protein) at low temperatures (0 degrees-2 degrees). Its hormone specificity, as measured by relative binding affinity (RBA) is spironolactone greater than prorenone greater than methyltrienolone greater than testosterone greater than progesterone greater than aldosterone greater than dexamethasone. In the same tissue preparation, specific spironolactone binding sites and classical mineralocorticoid receptor sites labelled with [3H]aldosterone differ in their thermal stability, binding parameters and hormone specificities, whereas their tissue distributions are similar. In conclusion, [3H]spironolactone binds specifically to kidney homogenates from adrenalectomized rats and these binding sites, apparently, are different from the classical mineralocorticoid receptors. The theoretical and practical aspects of this finding are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-2952
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2277-81
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Characterization of spironolactone binding sites distinct from aldosterone receptors in rat kidney homogenates.
pubmed:publicationType
Journal Article, In Vitro