Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1984-8-13
pubmed:abstractText
Genetic dissection of nucleoside transport in Leishmania donovani indicates that the insect vector form of these parasites possesses two biochemically distinct nucleoside transport systems. The first transports inosine, guanosine, and formycin B, and the second transports pyrimidine nucleosides and the adenosine analogs, formycin A and tubercidin. Adenosine is transported by both systems. A mutant, FBD5, isolated by virtue of its resistance to growth inhibition by 5 microM formycin B, cannot efficiently transport inosine, guanosine, or formycin B. This cell line is also cross-resistant to growth inhibition by a spectrum of cytotoxic analogs of inosine and guanosine. A second parasite mutant, TUBA5, isolated for its resistance to 20 microM tubercidin, cannot take up from the culture medium radiolabeled tubercidin, formycin A, uridine, cytidine, or thymidine. Both the FBD5 and the TUBA5 cell lines have about a 50% reduced capacity to take up adenosine, indicating that adenosine is transported by both systems. A tubercidin-resistant clonal derivative of FBD5, FBD5-TUB, has acquired the combined biochemical phenotype of each single mutant. The wild-type and mutant cell lines transport purine bases and uracil with equal efficiency. Mutational analysis of the relative growth sensitivities to cytotoxic nucleoside analogs and the selective capacities to take up exogenous radiolabeled nucleosides from the culture medium have enabled us to define genetically the multiplicity and substrate specificities of the nucleoside transport systems in L. donovani promastigotes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-307279, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-374403, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-4226168, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-4359485, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-468819, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-486094, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-500658, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-5011007, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-5545263, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-5660105, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-5893510, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-6572004, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-6604218, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-6631633, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-6681860, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-6971293, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-7073876, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-7110130, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-7150292, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-719006, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-7233189, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-7271805, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-7356667, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-7426045, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-758311, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-827549, http://linkedlifedata.com/resource/pubmed/commentcorrection/6234454-925380
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1013-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Genetic analysis of nucleoside transport in Leishmania donovani.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't