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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1984-1-7
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pubmed:databankReference | |
pubmed:abstractText |
Bovine cDNAs were cloned by using a mixture of 18-base-long synthetic deoxyribonucleotides as a hybridization probe. The longest cDNA clone (pBO-1) contained an 811-bp insert that included the 434 bp of the coding region corresponding to the C-terminal 144 amino acid residues of opsin peptide and the 377 bp of the 3'-untranslated region. The size of opsin mRNA was determined as 23 S by Northern blot hybridization. Bovine liver DNA gave rise to a single band of 2.8 kb, 1.1 kb and 7.9 kb each with Eco RI, Hind III and Bam HI, respectively, by Southern blot hybridization with pBO-1 as probe. Therefore, bovine opsin gene may occur once per haploid genome.
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pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
116
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
563-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:6228228-Amino Acid Sequence,
pubmed-meshheading:6228228-Animals,
pubmed-meshheading:6228228-Base Sequence,
pubmed-meshheading:6228228-Cattle,
pubmed-meshheading:6228228-DNA,
pubmed-meshheading:6228228-Eye Proteins,
pubmed-meshheading:6228228-Nucleic Acid Hybridization,
pubmed-meshheading:6228228-Retinal Pigments,
pubmed-meshheading:6228228-Rod Opsins
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pubmed:year |
1983
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pubmed:articleTitle |
Isolation and nucleotide sequence of a partial cDNA clone for bovine opsin.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|