Linked Life Data

6219467

Source:http://linkedlifedata.com/resource/pubmed/id/6219467

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-4-15
pubmed:abstractText
The growth of hemopoietic colonies in methylcellulose from bone marrow and spleen of CBA/Ca, DBA/2, BDF1, C57Bl/6, and NMRI mice was studied under stimulation by a mixed lymphocyte culture supernatant (MLC-BPA) which was necessary for the burst-forming unit E (BFU-E) growth in all strains. The number of pure and mixed BFU-E and non-BFU-E and their proportion varied between the strains; the BFU-E concentrations in the marrow were high in CBA/Ca and DBA/2 mice and low in C57B1/6. Furthermore, differences were seen in the requirement for serum and for erythropoietin. Optimal growth conditions were 4% fetal calf serum, 20% MLC-BPA, and 1 U erythropoietin/ml, but BFU-E growth was also obtained in the absence of serum. The differences in the BFU-E concentrations did not correlate with the numbers of colony-forming units S or the proportion of erythroblasts in the bone marrow smears.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0250-6793
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
177-88
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Differences in BFU-E growth in different mouse strains after stimulation with a mixed lymphocyte culture supernatant (MLC-BPA).
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't