rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1982-12-18
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pubmed:abstractText |
Dihydrocytochalasin B (H2CB) disrupts the actin structure of Swiss/3T3 mouse fibroblasts and inhibits the ability of serum growth factors to stimulate DNA synthesis in quiescent cultures. Low doses of H2CB (2-10 X 10(-7) M) added to serum-arrested cells reversibly block initiation of DNA synthesis by serum; by epidermal growth factor and insulin; or by epidermal growth factor, fibroblast growth factor and insulin. H2CB is effective only when added to cells within 8-10 hr after stimulation. Low doses of H2CB cause cell rounding and a loss of actin microfilament bundles, but they do not interfere with glucose or thymidine transport. These results suggest that stimulation of 3T3 cells involves at least one obligatory actin-mediated step. Transformed cells appear to obviate this step, for H2CB does not inhibit the entry into S phase of SV40-transformed or Moloney murine sarcoma virus-transformed 3T3 cells synchronized by mitotic shake-off.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochalasin B,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/dihydrocytochalasin B
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0092-8674
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
253-62
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:6215122-Actins,
pubmed-meshheading:6215122-Animals,
pubmed-meshheading:6215122-Biological Transport,
pubmed-meshheading:6215122-Blood,
pubmed-meshheading:6215122-Cell Line,
pubmed-meshheading:6215122-Cell Transformation, Viral,
pubmed-meshheading:6215122-Cytochalasin B,
pubmed-meshheading:6215122-Cytoskeleton,
pubmed-meshheading:6215122-DNA,
pubmed-meshheading:6215122-Dose-Response Relationship, Drug,
pubmed-meshheading:6215122-Epidermal Growth Factor,
pubmed-meshheading:6215122-Fibroblast Growth Factors,
pubmed-meshheading:6215122-Glucose,
pubmed-meshheading:6215122-Insulin,
pubmed-meshheading:6215122-Macromolecular Substances,
pubmed-meshheading:6215122-Mice,
pubmed-meshheading:6215122-Peptides,
pubmed-meshheading:6215122-Thymidine
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pubmed:year |
1982
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pubmed:articleTitle |
Dihydrocytochalasin B disorganizes actin cytoarchitecture and inhibits initiation of DNA synthesis in 3T3 cells.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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