Linked Life Data

6210265

Source:http://linkedlifedata.com/resource/pubmed/id/6210265

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1985-2-6
pubmed:abstractText
Determination of the nature of antigens towards which specific antibodies are directed has caused great difficulties in studies of the antigenic structure of the Mycoplasmatales. In immunoblotting, polypeptides are separated first by SDS polyacrylamide gel electrophoresis and transferred to cellulose nitrate electrophoretically. The resultant pattern is stained by enzyme-linked staining techniques. This permits direct detection of the antigenic specificities recognized by human and animal immune serum. For example, human convalescent sera from patients with Mycoplasma pneumoniae pneumonia recognize 2 to 7 polypeptides in M. pneumoniae, whereas human sera from patients with postpartum fever from whom Ureaplasma urealyticum has been isolated from the bloodstream detect 15 to 25 polypeptides. A comparison of M. pneumoniae with M. genitalium using rabbit antisera demonstrated that these two organisms show strong cross-reactions, although the organisms can be distinguished. Although certain antigens (epitopes) are destroyed in the procedure, it appears that about two-thirds of the polypeptides retain antigenicity. Immunoblotting provides a powerful means for identifying and subsequently fractionating antigens important to the human immune response.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-2180
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
908-11
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Immunoblotting for determination of the antigenic specificities of antibodies to the Mycoplasmatales.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.