Linked Life Data

6205134

Source:http://linkedlifedata.com/resource/pubmed/id/6205134

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-8-29
pubmed:abstractText
The substance P (SP) analog [tyrosine1, norleucine11]-substance P [( Tyr1, Nle11]-SP) was iodinated by the chloramine-T method to yield the mono iodinated derivative; both peptides were biologically active on the guinea-pig ileum SP receptor. Saturable, reversible binding of [125l-Tyr1, Nle11]-SP to a single class of noninteracting sites on the rat submaxillary gland homogenate was demonstrated with an affinity of 9.26 +/- 0.8 nM and a maximum binding of 15.7 +/- 1.25 or 250 +/- 20 fmol/mg of protein. The relative potencies of SP and its fragments SP(2-11) and SP(4-11), as measured by their IC50, are in agreement with their rank order in the salivation assay, whereas physalaemin was found apparently weaker than expected. However, their rank order in displacing [125I-Tyr1, Nle11]-SP was identical to their rank order in displacing [125I-Bolton-Hunter]-SP bound to mouse mesencephalic cells in primary culture.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-3565
pubmed:author
pubmed:issnType
Print
pubmed:volume
230
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
116-23
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Specific binding of a 125I-labeled substance P analog to rat submaxillary gland.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.