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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1984-7-31
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pubmed:abstractText |
A totally synthetic molecule (109-145 peptide) analogous to the beta-subunit carboxyl terminus was used as an antigen in the development of antibodies by the hybridoma technique. A monoclonal antibody (702 D7) specifically recognized the free native beta-human CG (beta hCG). 702 D7 was of the immunoglobulin G1 subclass and was directed against an antigenic site localized in a 10-amino acid sequence (109-118) or less. The recognition of an epitope located in the 109-118 region could explain the specific recognition of beta hCG observed with 702 D7, in contrast to monoclonal antibodies directed against a 118-145 region with a recognition of both beta hCG and whole hCG, as observed with a second monoclonal antibody (1032) to synthetic peptide. Immunohistochemical results and preliminary data obtained from the immunoradiometric assay show that 702 D7 provides a clinical tool for the detection of free beta-subunit secretion even at low concentrations, and could allow the study of this subunit or its metabolites produced by normal and tumoral cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0013-7227
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
115
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
330-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6203726-Animals,
pubmed-meshheading:6203726-Antibodies, Monoclonal,
pubmed-meshheading:6203726-Antibody Specificity,
pubmed-meshheading:6203726-Antigens,
pubmed-meshheading:6203726-Choriocarcinoma,
pubmed-meshheading:6203726-Chorionic Gonadotropin,
pubmed-meshheading:6203726-Chorionic Gonadotropin, beta Subunit, Human,
pubmed-meshheading:6203726-Histocytochemistry,
pubmed-meshheading:6203726-Humans,
pubmed-meshheading:6203726-Hybridomas,
pubmed-meshheading:6203726-Immunoenzyme Techniques,
pubmed-meshheading:6203726-Male,
pubmed-meshheading:6203726-Mice,
pubmed-meshheading:6203726-Mice, Inbred BALB C,
pubmed-meshheading:6203726-Peptide Fragments,
pubmed-meshheading:6203726-Radioimmunoassay,
pubmed-meshheading:6203726-Teratoma,
pubmed-meshheading:6203726-Testicular Neoplasms
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pubmed:year |
1984
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pubmed:articleTitle |
A monoclonal antibody against a synthetic peptide is specific for the free native human chorionic gonadotropin beta-subunit.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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