Linked Life Data

6200886

Source:http://linkedlifedata.com/resource/pubmed/id/6200886

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1984-5-2
pubmed:abstractText
We have characterized LPS+ murine lymphocytes by determining their surface phenotype using double-labeling immunofluorescence. In the spleen, 40% of cells were LPS+mu+ (B cells), 5% were LPS+Thy 1.2+ (T cells), and 5% were LPS+ cells bearing neither mu nor Thy 1.2 (null cells). Peripheral lymph nodes contained 11% LPS+ B cells and 2% LPS+ T cells, and mesenteric lymph nodes contained 15% LPS+ B cells and 3% LPS+ T cells. In contrast, the 4% LPS+ cells in Peyer's patches were all B cells, and the thymus contained 4% LPS+ T cells. Only the spleen contained LPS+ null cells. Within each lymphoid organ examined, the fraction of total lymphocytes identified as LPS+mu+, LPS+Ia+, or LPS+ delta + was similar, indicating that LPS+ B cells possessed the surface phenotype mu+Ia+ delta +, characteristic of a mature B cell. This conclusion was supported by the absence of LPS+mu+Ia+ delta + cells in newborn spleens. The fraction of mu+Ia+ delta + cells which also binds LPS was highest in spleen and lowest in Peyer's patches. Assuming that cells are, on the average, more mature in the progression from spleen to lymph nodes to Peyer's patches, it would appear that LPS+ cells are a less mature fraction of the mu+Ia+ delta + pool, distinguished by the presence of an LPS binding site or receptor. These data illustrate selective binding of LPS predominantly to mature B cells, but also to small numbers of null cells and T cells. The relationship of this binding to cell activation is discussed by considering the characteristics of cells which can be activated by LPS to clonal growth or differentiation under appropriate conditions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0037-9727
pubmed:author
pubmed:issnType
Print
pubmed:volume
175
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
458-67
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Surface phenotype of LPS-binding murine lymphocytes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't