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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1984-2-22
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pubmed:abstractText |
The generation of histamine releasing activity (HRA) from human basophils in fresh serum by tetanus toxoid (Te)/anti-Te complexes or by zymosan can be modulated through introduction of incremental amounts of proteins B and H of the alternative complement pathway. Serum treated at 50 degrees in order to abolish alternative pathway-mediated haemolytic activity, lost 90% of its capacity to generate HRA upon addition of Te/aTe; such loss could be reversed through additions of purified B. Amounts of B sufficient to restore normal alternative pathway haemolytic activity also restored HRA induced by Te/aTe; as little as a 33% increase above the normal serum concentration of B increased the capacity to support Te/aTe induced HRA by a factor of 1.4. In contrast, additions of incremental doses of purified H to fresh serum reduced generation of HRA by both Te/aTe and zymosan. Total inhibition was achieved by increasing the serum H concentration by 12.5-30%; further increases of H up to 200% again permitted HRA generation induced by immune complexed aTe. H also inhibited Te/aTe induced HRA in a serum heated at 50 degrees but only 30% inhibition of HRA could be achieved over a range of H inputs up to 187% above normal serum concentration. Additions of H also inhibited HRA generation in fresh serum when induced with plain or C3b-coated zymosan (Z) particles. By increasing the serum concentration of H from 12.5 to 125%, dose-dependent inhibition of HRA generation was observed; the H input necessary to suppress 48% of HRA generation was ten times higher when HRA was generated by Z-C3b than by plain zymosan. Thus, the complement-dependent generation of HRA from fresh serum strongly depends on modest variations in the concentrations of the two regulatory proteins B and H of the alternative complement pathway, suggesting their direct effect on generation of anaphylatoxins C3a and C5a.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-1067618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-1141690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-266208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-279010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-301546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-342601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-4202731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-4632688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-46892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-4734384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-48505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-56398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-565382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-6115248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-6170591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-6211407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-6223741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-62817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-6461451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-6800960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-7086356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-7108223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-7204978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-7264302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-7360115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-762425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-804510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-87438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-90082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-90083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197357-950465
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anaphylatoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Antigen-Antibody Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Complement C3b,
http://linkedlifedata.com/resource/pubmed/chemical/Complement C3b Inactivator Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Complement Factor B,
http://linkedlifedata.com/resource/pubmed/chemical/Complement Factor H,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Tetanus Toxoid,
http://linkedlifedata.com/resource/pubmed/chemical/Zymosan,
http://linkedlifedata.com/resource/pubmed/chemical/complement factor H, human
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
51
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
123-31
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6197357-Anaphylatoxins,
pubmed-meshheading:6197357-Antigen-Antibody Complex,
pubmed-meshheading:6197357-Basophils,
pubmed-meshheading:6197357-Complement Activation,
pubmed-meshheading:6197357-Complement C3b,
pubmed-meshheading:6197357-Complement C3b Inactivator Proteins,
pubmed-meshheading:6197357-Complement Factor B,
pubmed-meshheading:6197357-Complement Factor H,
pubmed-meshheading:6197357-Complement Pathway, Alternative,
pubmed-meshheading:6197357-Enzyme Precursors,
pubmed-meshheading:6197357-Histamine Release,
pubmed-meshheading:6197357-Humans,
pubmed-meshheading:6197357-Peptide Biosynthesis,
pubmed-meshheading:6197357-Tetanus Toxoid,
pubmed-meshheading:6197357-Zymosan
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pubmed:year |
1984
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pubmed:articleTitle |
Control of immune complex and zymosan-mediated anaphylatoxin generation by proteins B and H of the alternative complement pathway.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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