Linked Life Data

6175723

Source:http://linkedlifedata.com/resource/pubmed/id/6175723

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-6-21
pubmed:abstractText
An amidolytic assay using the chromogenic substrate tosyl-glycyl-prolyl-lysine-4-nitranilide acetate (Chromozym PL) has been studied for the determination of alpha 1-antitrypsin in serum and in cerebrospinal fluid (CSF). It was found that binding of trypsin to alpha 2-macroglobulin has to be taken into consideration when alpha 1-antitrypsin is determined by an amidolytic assay. In serum this accounts for 7.4 +/- 3.2% and in CSF 2.8 +/- 1.8% of the total antitrypsin capacity. The reference range of alpha 1-antitrypsin in sera was found to be x = 60.3 +/- 20.8 kIU/l, and in CSF mean = 186 +/- 99 IU/l. Within-run precision showed a C.V. of 1.36% in sera, and a C.V. of 1.86% in CSF. There was a good correlation with immunochemical methods: r = 0.946 in 65 sera (p less than 0.001) and r = 0.986 in 55 samples of CSF (p less than 0.001).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0340-076X
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
57-60
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
An amidolytic assay for determination of alpha 1-antitrypsin in serum and in cerebrospinal fluid.
pubmed:publicationType
Journal Article, Comparative Study