Linked Life Data

6169785

Source:http://linkedlifedata.com/resource/pubmed/id/6169785

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 2
pubmed:dateCreated
1981-12-15
pubmed:abstractText
A persistent infection of the human MCF-7 cell line (MCF-7RV) was established with the DBS strain of rubella virus at a low multiplicity of infections. Fluorescent antibody staining revealed that 100% of the cells were positive for rubella antigens. The infected cells were refractory to superinfection with vesicular stomatitis virus (VSV) but were susceptible to herpes simplex virus type 2 (HSV-2). No interferon could be detected in infected cell culture fluid, and continuous passage in the presence of antibody did not lead to a decrease in the percentage of infected cells. Virus production in the persistently infected cells represented a 5- to 10-fold increase over primary infection. Plaque assays at 30 and 39 degree C of the virus produced at 37 degree C revealed the presence of temperature-sensitive (ts) mutants. If MCF-7RV cells were maintained at 30 degree C, significant increases in virus production were observed, leading to cytopathic effect and destruction of the monolayer. If maintained at 39 degree C, MCF-7RV cells produced less virus and demonstrated normal morphology. These data suggest that the naturally selected population of ts mutants being produced by these cells represents the mechanism by which persistence is maintained.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
321-8
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Characteristics of a persistent rubella infection in a human cell line.
pubmed:publicationType
Journal Article