Linked Life Data

6168449

Source:http://linkedlifedata.com/resource/pubmed/id/6168449

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-11-18
pubmed:abstractText
The biochemical composition of any isolated organelle is affected by the agent(s) to which it has been exposed during the isolation and purification procedures. Many agents are known to extract molecular components either selectively or nonselectively. The protein composition of isolated chromosomes exposed to fluorochromes must be examined before proceeding to the biochemical analysis of the proteins from individual chromosomes purified by flow cytometry techniques. We have examined the polyacrylamide gel electrophoresis protein profiles from from metaphase chromosome exposed to the fluorochromes and staining conditions commonly used for flow cytometry. We find that the electropherograms of the protein of metaphase chromosomes which have been exposed to various fluorochromes are indistinguishable from controls. The analysis of proteins from individual chromosomes sorted by flow cytometry can now be undertaken.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0196-4763
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
18-20
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Proteins from metaphase chromosomes treated with fluorochromes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.