Linked Life Data

6165641

Source:http://linkedlifedata.com/resource/pubmed/id/6165641

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-8-10
pubmed:abstractText
Studies were performed to determine relationships among Na+, K+-ATPase, the transmucosal Na+ gradient, and bile-acid transport in metabolically viable cells isolated from rat ileum. Incubation of cells with 0, 10(-6), 10(-5), 10(-4) and 10(-3) M ouabain resulted, respectively, in a 0, 10.3, 42.1, 97.0, and 100% decrease in glycocholate uptake and a 0, 10.7, 46.4, 76.8, and 100% decrease in Na+, K+-ATPase activity. Thus, one-half maximal inhibition of glycocholate uptake and Na+, K+-ATPase activity occurred at 5.5 x 10(-5) M and 1.7 x 10(-5) M ouabain, respectively. A change in glycocholate uptake was correlated with a change in Na+, K+-ATPase activity after daily injections of methylprednisolone. After 4 days treated animals showed a 26% and 36% increase in glycocholate uptake and Na+, K+-ATPase activity, respectively, over pair-fed saline-treated controls (p less than 0.001). Methylprednisolone did not significantly alter the activity of (Mg++)-ATPase when compared with controls (p greater than 0.05). Glycocholate uptake was reduced by the omission of Na+ from the incubation medium. Preincubation of cells at 37 degrees C with gramicidin D, 10 micrograms/ml, to alter membrane permeability to Na+, resulted in a significant rise in cell Na+ (p less than 0.01) and a significant fall in glycocholate uptake from values in untreated cells (p less than 0.01) to approach values for glycocholate uptake at 0 degrees C. These data suggest that Na+, K+-ATPase may play a role in a bile-acid uptake into ileal cells possibly by maintaining a Na+ electrochemical potential gradient for coupled Na+-bile-acid transport.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0016-5085
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
54-60
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Studies of relationship among bile-acid uptake, Na+, K+-ATPase, and Na+ gradient in isolated cells from rat ileum.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.