|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
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|
pubmed:dateCreated |
1981-7-23
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|
pubmed:abstractText |
Growth and interferon production by human lymphoblastoid cells (Namalva) have been achieved by using medium with a complete substitution of human albumin for fetal bovine serum. Medium containing 0.3% human albumin supported exponential cell growth (minimum doubling time, 20 h) in Bilthoven 10-liter fermentors. Interferon induction with Sendai virus resulted in interferon yields of 0.5 x 10(4) to 1.0 x 10(4) interferon units per 10(6) Namalva cells per ml in RPMI 1640 medium containing 0.15 to 0.3% human albumin. Both cell growth and interferon production in medium containing human albumin were comparable to growth and interferon production in the same medium containing 10 and 5% fetal bovine serum, respectively.
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|
pubmed:commentsCorrections |
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0066-4804
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
18
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
930-2
|
|
pubmed:dateRevised |
2009-11-18
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|
pubmed:meshHeading |
|
|
pubmed:year |
1980
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|
pubmed:articleTitle |
Replacement of animal serum proteins by human albumin for growth and interferon production by Namalva cells.
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|
pubmed:publicationType |
Journal Article
|
