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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1981-1-26
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pubmed:abstractText |
Papain-solubilized tumour-specific antigens from the aminoazo dye-induced rat hepatoma D23 were purified by a combination of lectin affinity and immunoadsorbent column chromatography. Isolated antigens were radio-iodinated using three procedures and analysed for their reaction with specific antibodies in syngeneic immune sera by double-antibody co-precipitation tests and by the rebinding of labelled antigens to specific and non-relevant antibodies immobilized on Sepharose-4B. Soluble hepatoma D23-specific antigens were labile to radiolabelling, and for optimal retention of serological reactivity it was necessary to protect the antigenic determinant by performing the chloramine T method of iodination with antigen bound to the immunoadsorbent followed by elution from the solid phase with 3M NaSCN. Immunoadsorption chromatography indicated that one consequence of radiolabelling hepatoma D23-specific antigen with 125I was a reduction in the affinity of the labelled antigen for its syngeneic specific antibody.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-1259913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-169868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-197192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-221923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-340027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-4160044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-4364263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-4758368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-5563761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-58946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-81850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-89101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-892137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158964-975111
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0007-0920
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
41
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
716-23
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:6158964-Animals,
pubmed-meshheading:6158964-Antibody Specificity,
pubmed-meshheading:6158964-Antigens, Neoplasm,
pubmed-meshheading:6158964-Chemical Precipitation,
pubmed-meshheading:6158964-Chromatography, Ion Exchange,
pubmed-meshheading:6158964-Epitopes,
pubmed-meshheading:6158964-Immunoglobulin G,
pubmed-meshheading:6158964-Immunosorbent Techniques,
pubmed-meshheading:6158964-Iodine Radioisotopes,
pubmed-meshheading:6158964-Isotope Labeling,
pubmed-meshheading:6158964-Liver Neoplasms, Experimental,
pubmed-meshheading:6158964-Rats
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pubmed:year |
1980
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pubmed:articleTitle |
Radioiodination of rat hepatoma-specific antigens and retention of serological reactivity.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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