Linked Life Data

6120262

Source:http://linkedlifedata.com/resource/pubmed/id/6120262

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1982-4-22
pubmed:abstractText
The fluorescence-promoting effects of sodium, magnesium and aluminum ions in the histochemical formaldehyde (FA) reaction with catecholamines and indoleamines have been studied in protein models. The positive effects of aluminum and magnesium salts on the monoamine fluorescence yields, seen in tissue, could partly be reproduced in a simple protein matrix. The fluorescence-promoting potency was greatest for aluminum, moderate for magnesium and small for sodium. The aluminum effect was markedly concentration-dependent, with an optimum at a concentration of 5-10 mM in the model solution. Optimum pH was around 3.8. Experiments with synthetic fluorophores indicate that the principal action of the metal ions is on the fluorescence properties of the monoamine fluorophores rather than on the yield of fluorophores in the reaction of the monoamines with FA. The presence of aluminum ions in the models thus causes both increases in the fluorescence efficiency of the fluorophores as well as changes in their spectral properties. The direct effects on the monoamine fluorophores cannot, however, account for all effects of the metal salts seen in tissue. It is suggested that aluminum and magnesium ions, in addition, can act as acid catalysts in the FA-monoamine reactions, and that the metal salts have a direct effect on the tissue environment, leading to a 'locking-in' of the intracellular monoamines.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
301-18
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
The aluminum-formaldehyde (ALFA) histofluorescence method for improved visualization of catecholamines and indoleamines. 2. Model experiments.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't