Linked Life Data

6114750

Source:http://linkedlifedata.com/resource/pubmed/id/6114750

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-10-29
pubmed:abstractText
Poly(C) and heparin at low concentrations (1 microgram/ml) prevent the RNA synthesis termination protein rho from functioning during the biosynthesis of RNA from bacteriophage T7 DNA catalyzed by Escherichia coli RNA polymerase. Both of these polyanions inhibit the binding of rho to isolated T7 RNA. Heparin also inhibits rho ATPase when isolated RNA transcripts are used as cofactors. It is concluded that the polyanions inhibit termination by binding to the site on rho that is normally used for the initial interaction with a nascent RNA transcript in the rho-mediated release of RNA. Since one of the inhibitors, poly(C), is itself a potent activator for rho ATPase, it is also concluded that the ATP hydrolysis step that is required for rho termination has to be coupled to an action of rho on the RNA molecule to be released from the transcription complex.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
655
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
96-101
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Inhibition of the action of Escherichia coli transcription termination protein rho by poly(C) and heparin.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.