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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-3
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pubmed:dateCreated |
1985-3-29
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pubmed:abstractText |
A strategy for gene cloning in the cyanobacterium Anacystis nidulans R2 was developed which made use of a gene library constructed in a shuttle cosmid vector. The method involved phenotypic complementation of mutants with pooled cosmid DNA. The development of the procedure and its application to the cloning of a third gene involved in nitrate reduction are described.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
109-16
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6098517-Bacterial Proteins,
pubmed-meshheading:6098517-Bacteriophage lambda,
pubmed-meshheading:6098517-Cloning, Molecular,
pubmed-meshheading:6098517-Cyanobacteria,
pubmed-meshheading:6098517-DNA, Bacterial,
pubmed-meshheading:6098517-DNA, Recombinant,
pubmed-meshheading:6098517-Escherichia coli,
pubmed-meshheading:6098517-Genes,
pubmed-meshheading:6098517-Genes, Bacterial,
pubmed-meshheading:6098517-Genetic Complementation Test,
pubmed-meshheading:6098517-Genetic Vectors,
pubmed-meshheading:6098517-Nitrate Reductases,
pubmed-meshheading:6098517-Plasmids
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pubmed:year |
1984
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pubmed:articleTitle |
Cloning of a third nitrate reductase gene from the cyanobacterium Anacystis nidulans R2 using a shuttle cosmid library.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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