Linked Life Data

6092402

Source:http://linkedlifedata.com/resource/pubmed/id/6092402

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1984-12-3
pubmed:abstractText
Cation-exchange high-performance liquid chromatography (HPLC) was used to increase the sensitivity and specificity of the radioimmunoassay of plasma beta-endorphin. Proteins were precipitated from a 0.5 to 2.5 ml sample of plasma with 60% acetonitrile at pH 4.7. The supernatant was subjected to cation-exchange HPLC. Gradient elution with volatile buffers was used to separate beta-endorphin from beta-lipotropin. The beta-endorphin fraction (1.8 ml) was concentrated by lyophilization and subjected to radioimmunoassay. In healthy pregnant women at labour plasma concentration of beta-endorphin varied from 105 to 403 pg/ml. In healthy non-pregnant women plasma concentration of beta-endorphin was low, exceeding the detection limit (4 pg/ml) of the assay in only one of the 7 subjects studied.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
297
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
399-403
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Rapid extraction and separation of plasma beta-endorphin by cation-exchange high-performance liquid chromatography.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't