Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1985-4-16
pubmed:abstractText
In Mycoplasma capricolum 75% of the total cell phosphate is found in the nucleic acids and polyphosphate fraction. The cold acid soluble fraction consisted primarily of orthophosphate (40 mM) in resting organisms. In metabolizing cells, phosphate esters, probably organophosphates, represented the largest part of that fraction (40 mM) whose content in orthophosphate is lowered to 10-15 mM. Phosphate is accumulated against its concentration gradient after its entry as orthophosphate. It is taken up by the cell by two different, energy-stimulated transport systems. The response of both systems to metabolizable substrates resembles what is described for other microorganisms. The high affinity system had a Km of 12.8 microM and the low affinity system had a Km of 1.1 mM when cells were grown in a phosphate-poor medium. Addition of phosphate (30 mM) to that medium modified both system kinetic properties. Inhibition of phosphate influx by arsenate is limited to the energy-stimulated portion of the influx in both systems.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0714-7511
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1041-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Phosphate distribution and transport in mycoplasma.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't