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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1976-9-1
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pubmed:abstractText |
The inhibitory effect of tRNA on yeast 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase (EC 4.1.2.15) has been reinvestigated. From earlier studies the inhibition by tRNAPhe appeared to be quite specific. This study shows that tRNAPhe is indeed a potent inhibitor but so is unfractionated tRNA, as well as ribosomal RNA and heparin. Complete digestion to mononucleotides relieves the inhibition. Since the enzyme requires a metal ion (Co2+) we suggest that the RNA and heparin are inhibitory by virtue of their capacity to chelate the Co2+.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
435
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
21-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6062-3-Deoxy-7-Phosphoheptulonate Synthase,
pubmed-meshheading:6062-Aldehyde-Lyases,
pubmed-meshheading:6062-Cobalt,
pubmed-meshheading:6062-Enzyme Activation,
pubmed-meshheading:6062-Hydrogen-Ion Concentration,
pubmed-meshheading:6062-Kinetics,
pubmed-meshheading:6062-Magnesium,
pubmed-meshheading:6062-Manganese,
pubmed-meshheading:6062-Nickel,
pubmed-meshheading:6062-Phenylalanine,
pubmed-meshheading:6062-RNA, Transfer,
pubmed-meshheading:6062-Saccharomyces cerevisiae
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pubmed:year |
1976
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pubmed:articleTitle |
A nonspecific inhibitory effect of tRNA on the activity of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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