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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1966-10-15
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pubmed:abstractText |
1. Flash illumination of a suspension of frog rod outer segments or rhodopsin solution in contact with a platinum electrode produces a rapidly developing negative displacement of potential of the electrode (with respect to a reversible electrode).2. The amplitude of the potential change varies inversely with the H(+) buffering capacity of the medium. It is inferred that the response is due to an uptake of H(+) by the rod outer segments or rhodopsin, with the platinum surface acting as a pH electrode.3. Determination of the action spectrum shows that the response depends on the absorption of light by rhodopsin.4. In frog rods one acid-binding group with a pK of about 7.9 is produced for each molecule of rhodopsin bleached, consistent with a rhodopsin concentration in frog rods of 1.7 mM.5. It is suggested that the time course of the response with rhodopsin solutions reflects the kinetics of the conversion of metarhodopsin I to metarhodopsin II.6. A slower time course of voltage change observed for suspensions of outer segments is attributable to the time required for the diffusion of H(+) buffer out of the rods.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/5945249-13018143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5945249-13118108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5945249-13322011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5945249-13346044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5945249-13525671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5945249-13627858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5945249-13901700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5945249-14002749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5945249-14080814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5945249-14085016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5945249-15403120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5945249-16995211
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
183
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
211-24
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1966
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pubmed:articleTitle |
Rapid hydrogen ion uptake of rod outer segments and rhodopsin solutions on illumination.
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pubmed:publicationType |
Journal Article,
In Vitro
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