Linked Life Data

582596

Source:http://linkedlifedata.com/resource/pubmed/id/582596

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1979-11-21
pubmed:abstractText
The membranes of Limulus (horseshoe crab) sperm were examined before and during the acrosomal reaction by using the technique of freeze-fracturing and thin sectioning. We focused on three areas. First, we examined stages in the fusion of the acrosomal vacuole with the cell surface. Fusion takes place in a particle-free zone which is surrounded by a circlet of particles on the P face of the plasma membrane and an underlying circlet of particles on the P face of the acrosomal vauole membrane. These circlets of particles are present before induction. Up to nine focal points of fusion occur within the particle-free zone. Second, we describe a system of fine filaments, each 30 A in diameter, which lies between the acrosomal vacuole and the plasma membrane. These filaments change their orientation as the vacuole opens, a process that takes place in less than 50 ms. Membrane particles seen on the P face of the acrosomal vacuole membrane change their orientation at the same time and in the same way as do the filaments, thus indicating that the membrane particles and filaments are probably connected. Third, we examined the source and the point of fusion of new membrane needed to cover the acrosomal process. This new membrane is almost certainly derived from the outer nuclear envelope and appears to insert into the plasma membrane in a particle-free area adjacent to an area rich in particles. The latter is the region where the particles are probably connected to the cytoplasmic filaments. The relevance of these observations in relation to the process of fertilization of this fantastic sperm is discussed.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/582596-100501, http://linkedlifedata.com/resource/pubmed/commentcorrection/582596-1060120, http://linkedlifedata.com/resource/pubmed/commentcorrection/582596-1097941, http://linkedlifedata.com/resource/pubmed/commentcorrection/582596-1110768, http://linkedlifedata.com/resource/pubmed/commentcorrection/582596-1195351, http://linkedlifedata.com/resource/pubmed/commentcorrection/582596-319098, http://linkedlifedata.com/resource/pubmed/commentcorrection/582596-4204034, http://linkedlifedata.com/resource/pubmed/commentcorrection/582596-4507616, http://linkedlifedata.com/resource/pubmed/commentcorrection/582596-4596345, http://linkedlifedata.com/resource/pubmed/commentcorrection/582596-830653, http://linkedlifedata.com/resource/pubmed/commentcorrection/582596-856830, http://linkedlifedata.com/resource/pubmed/commentcorrection/582596-885915
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
229-53
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
Membrane events in the acrosomal reaction of Limulus sperm. Membrane fusion, filament-membrane particle attachment, and the source and formation of new membrane surface.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.