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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1969-9-19
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pubmed:abstractText |
Present techniques for the estimation of platelet ascorbic acid allow interference by other substances in the sample. A new and more specific method of analysis is presented. The proposed method owes its increased specificity to resolution of the extract by thin-layer chromatography. By this means ascorbic acid is separated from other reducing substances present. The separated ascorbic acid is eluted from the thin layer and estimated by a new and very sensitive procedure: ascorbic acid is made to react with ferric chloride and the ferrous ions so formed are estimated spectrophotometrically by the coloured derivative which they form with tripyridyl-Striazine. Results obtained with normal blood platelets were consistently lower than simultaneous determinations by the dinitrophenylhydrazine (DNPH) method.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9746
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
453-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:5798633-Ascorbic Acid,
pubmed-meshheading:5798633-Blood Platelets,
pubmed-meshheading:5798633-Chlorides,
pubmed-meshheading:5798633-Chromatography, Thin Layer,
pubmed-meshheading:5798633-Iron,
pubmed-meshheading:5798633-Methods,
pubmed-meshheading:5798633-Oxidation-Reduction,
pubmed-meshheading:5798633-Phenylhydrazines,
pubmed-meshheading:5798633-Spectrophotometry,
pubmed-meshheading:5798633-Triazines
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pubmed:year |
1969
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pubmed:articleTitle |
New method for the estimation of platelet ascorbic acid.
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pubmed:publicationType |
Journal Article
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