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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
1976-5-25
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pubmed:abstractText |
The introduction of a fluorescent staining technique for identification of Y chromosome made possible the distinction of X- and Y-bearing human spermatozoa. Using this fluorescence analysis the isolation of Y- and X-bearing human spermatozoa were investigated employing two different methods for separation of spermatozoa. 1. The migration of human spermatozoa in cervical mucus obtained from women shortly before mid-cycle was studied, using an in-vitro method for horizontal sperm penetration. Basing on the progressive sperm motility in cervical mucus, a fraction rich in Y-bearing spermatozoa in the frontal zone of sperm migration was found. 2. Gradient centrifugation at different rotor speeds using a discontinous sucrose gradient has been employed for separation of human spermatozoa. A significant trend for separation of Y- and X-bearing spermatozoa was demonstrated. The results were shortly discussed.
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pubmed:language |
ger
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
0044-4197
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
97
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
408-12
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pubmed:dateRevised |
2008-2-11
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pubmed:meshHeading |
pubmed-meshheading:56829-Centrifugation, Density Gradient,
pubmed-meshheading:56829-Cervix Mucus,
pubmed-meshheading:56829-Male,
pubmed-meshheading:56829-Methods,
pubmed-meshheading:56829-Sex Chromosomes,
pubmed-meshheading:56829-Sperm Motility,
pubmed-meshheading:56829-Sperm Transport,
pubmed-meshheading:56829-Spermatozoa,
pubmed-meshheading:56829-Staining and Labeling
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pubmed:year |
1975
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pubmed:articleTitle |
[Problem in the partial separation of X- and Y-carrying human spermatozoa].
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pubmed:publicationType |
Journal Article,
In Vitro,
English Abstract
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