Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1978-5-17
pubmed:abstractText
The synthesis of a fluorescent colchicine derivative permits the localization of colchicine-binding receptors in cells. Fluorescein colchicine (FC) was prepared by the addition of fluorescein isothiocyanate to deacetyl colchicine. The product, FC, was separated from the reactants by thin-layer chromatography (TLC). The purity of FC was demonstrated by TLC, UV spectral analysis, and analysis of the kinetics of photodecomposition. FC inhibited [3H] colchicine binding to purified brain tubulin. The biological activity of FC was compared to the activity of unlabeled colchicine on mitosis, motility, secretion, and myogenesis. The effects of FC were identical to those of unlabeled colchicine in all biological systems tested. The results demonstrate that FC may be substituted for colchicine in biological experiments without significant loss in specificity or effectiveness.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-1132413, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-1141240, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-1141379, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-1170172, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-1176440, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-125062, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4109310, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4126253, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4132067, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4261039, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4339877, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4402005, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4426914, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4514990, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4557309, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4584367, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4809244, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4811890, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4842032, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4881355, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4882889, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-4923996, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-5065219, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-5586460, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-5664223, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-5700666, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-5775787, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-5959471, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-5971010, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-6035644, http://linkedlifedata.com/resource/pubmed/commentcorrection/564913-6058222
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
619-27
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Fluorescein colchicine. Synthesis, purification, and biological activity.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.