Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1977-9-29
pubmed:abstractText
Complementary strand-specific adenovirus DNA, either full length or from restriction enzyme cleavage fragments, was used to estimate the fractional representation and abundance of viral sequences in two adenovirus type 2 (Ad2)-transformed rat cell lines, A2F19 and A2T2C4. The reassociation method introduced is based on the linear relationship, after exhaustive hybridization, between the inverted fraction of hybrid DNA and the molar ratio of probe to cellular DNA in the reaction mixture. The amount of viral DNA in A2F19 cells represents 12 to 14% of the viral genome at a level of around seven copies per diploid cell equivalent. For the cell line A2T2C4, the pattern of integrated viral DNA sequences is more complex. With full-length Ad2 DNA strands as a probe, about 56% of the probe was represented in cellular DNA. When each of the four BamHI fragment strands of Ad2 DNA was used as a probe, the fraction of the viral DNA present also amounted to around 56% with one to five copies from different regions of the viral genome. The results demonstrate the advantage of using strand-specific viral DNA as a probe in reassociation analysis with denatured cell DNA. The method should be useful in any system in which complementary strand separation of viral DNA sequences can be achieved.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-1057470, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-1099215, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-1182803, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-1263260, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-169053, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-169095, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-185395, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-4325134, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-4342195, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-4354250, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-4355929, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-4427381, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-4473553, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-4567334, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-4612107, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-4613857, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-4734785, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-4810778, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-50907, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-948869, http://linkedlifedata.com/resource/pubmed/commentcorrection/560494-988193
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-35
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Reassociation of complementary strand-specific adenovirus type 2 DNA with viral DNA sequences of transformed cells.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.