Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1971-7-15
pubmed:abstractText
Previous work has suggested that intracellular proteolysis may play a role in lymphocyte stimulation. An inhibitor of proteolysis, epsilon amino caproic acid (EACA) was studied for its effect on the lymphocyte response to phytohemagglutinin (PHA). EACA was found to inhibit several parameters of lymphocyte stimulation (e.g. DNA, RNA, and protein synthesis as well as alterations in morphology) This inhibition was not due to diminished cellular viability and did not permanently impair the capacity of the lymphocyte to subsequently respond to PHA. Additionally, there was no evidence that this inhibition was due to other possible effects of EACA, such as alterations in Na(+) - K(+) transport, competitive amino acid deprivation or interference with PHA binding. Moreover, the inhibitors of proteolysis, tosyl arginine methyl ester (TAME), tosyl lysine chloromethyl ketone (TLCK), and tosyl phenyl-alanine chloromethyl ketone (TPCK), were also shown to inhibit lymphocyte stimulation.EACA was most effective when added during the first 24 hr of stimulation. Therefore, these experiments support the hypothesis that proteolysis is an essential step in the early phase of lymphocyte activation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-13293190, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-13654273, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-14012159, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-14209764, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-14226518, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-4239437, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-4299844, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-4314861, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-4910267, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5219687, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5254270, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5305809, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5358342, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5459892, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5645728, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5656398, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5656399, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5659654, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5776470, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5780797, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5780847, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5789659, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5824458, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5839195, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5891322, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5919445, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5931380, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-5969294, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-6022318, http://linkedlifedata.com/resource/pubmed/commentcorrection/5578231-6051393
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Aminocaproic Acids, http://linkedlifedata.com/resource/pubmed/chemical/Arginine, http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Carbon Isotopes, http://linkedlifedata.com/resource/pubmed/chemical/DNA, http://linkedlifedata.com/resource/pubmed/chemical/Esters, http://linkedlifedata.com/resource/pubmed/chemical/Ketones, http://linkedlifedata.com/resource/pubmed/chemical/Lectins, http://linkedlifedata.com/resource/pubmed/chemical/Leucine, http://linkedlifedata.com/resource/pubmed/chemical/Lysine, http://linkedlifedata.com/resource/pubmed/chemical/Phenylalanine, http://linkedlifedata.com/resource/pubmed/chemical/RNA, http://linkedlifedata.com/resource/pubmed/chemical/Thymidine, http://linkedlifedata.com/resource/pubmed/chemical/Uridine
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1206-17
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:5578231-Aminocaproic Acids, pubmed-meshheading:5578231-Arginine, pubmed-meshheading:5578231-Blood Proteins, pubmed-meshheading:5578231-Carbon Isotopes, pubmed-meshheading:5578231-Culture Techniques, pubmed-meshheading:5578231-DNA, pubmed-meshheading:5578231-Depression, Chemical, pubmed-meshheading:5578231-Drug Antagonism, pubmed-meshheading:5578231-Esters, pubmed-meshheading:5578231-Humans, pubmed-meshheading:5578231-Ketones, pubmed-meshheading:5578231-Lectins, pubmed-meshheading:5578231-Leucine, pubmed-meshheading:5578231-Lymphocyte Activation, pubmed-meshheading:5578231-Lymphocytes, pubmed-meshheading:5578231-Lysine, pubmed-meshheading:5578231-Phenylalanine, pubmed-meshheading:5578231-RNA, pubmed-meshheading:5578231-Stimulation, Chemical, pubmed-meshheading:5578231-Thymidine, pubmed-meshheading:5578231-Uridine
pubmed:year
1971
pubmed:articleTitle
The effect of epsilon amino caproic acid and other inhibitors of proteolysis upon the response of human peripheral blood lymphocytes to phytohemagglutinin.
pubmed:publicationType
Journal Article
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