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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1970-5-5
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pubmed:abstractText |
1. Liver RNA synthesis was studied within 24h after whole-body X-irradiation of guinea pigs that had been starved for 22-24h. 2. Microsomal RNA was labelled in vivo for 3h with [(14)C]orotic acid and the isolated labelled RNA was fractionated by sucrose-density-gradient centrifugation. Incorporation was 50-100% higher between 3 and 12h after 2000rd X-irradiation and at 22h was not elevated any further. Whole nuclear RNA was labelled with [(14)C]orotic acid for 15min. At 5h after irradiation the incorporation showed a 50-100% increase. Incorporation increased in all types of RNA studied. 3. The RNA phosphorus/DNA phosphorus ratio of whole liver gradually increased after X-irradiation. Maximal increase was found between 24 and 36h, which corresponds to a value about 40% above that of the starved control. The RNA phosphorus content of isolated ribonucleoproteins obtained from various cell fractions of the liver was similarly increased after X-irradiation. 4. Liver microsomes were obtained from X-irradiated and control animals. Microsomes were incubated in vitro with [(14)C]phenylalanine in the presence and absence of polyuridylic acid. After the incubation the microsomes were fractionated by sucrose-density-gradient centrifugation. The polyuridylic acid enhancement was twice as great in the microsomes of the control preparation as in the irradiated one. The experiment demonstrated a higher saturation of microsomes by endogenous messenger after X-irradiation. 5. RNA polymerase activity of the purified nuclear preparation was assayed. The activity of the Mg(2+)-dependent RNA polymerase activity was 50 and 200% respectively above the control values at 6 and 9h after X-irradiation. 6. Animals were treated with actinomycin D shortly before X-irradiation. This treatment abolished the radiation-induced enrichment of polyribosomes and the increase of protein-synthesizing activity. The effect of X-irradiation on the transcription of the genetic code of the liver is discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-13037771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-13076983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-13293190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-13315239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-13337583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-13375653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-13460973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-13625865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-13689431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-13756977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-13877968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-13954377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-13978666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-14110449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-14126581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-14151365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-14153520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-14163775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-14172996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-14262461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-14324825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-14367363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-4170424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-4284460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-4387148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-4962085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-5683502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-5835791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-5838072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-5880015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-5905134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-5964035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-5971434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5435693-6032987
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
116
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
503-14
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:5435693-Animals,
pubmed-meshheading:5435693-Carbon Isotopes,
pubmed-meshheading:5435693-Cell Nucleus,
pubmed-meshheading:5435693-Centrifugation, Density Gradient,
pubmed-meshheading:5435693-Dactinomycin,
pubmed-meshheading:5435693-Genetic Code,
pubmed-meshheading:5435693-Guinea Pigs,
pubmed-meshheading:5435693-Liver,
pubmed-meshheading:5435693-Male,
pubmed-meshheading:5435693-Microsomes, Liver,
pubmed-meshheading:5435693-Orotic Acid,
pubmed-meshheading:5435693-Phenylalanine,
pubmed-meshheading:5435693-RNA,
pubmed-meshheading:5435693-RNA Nucleotidyltransferases,
pubmed-meshheading:5435693-Radiation Effects
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pubmed:year |
1970
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pubmed:articleTitle |
The effect of whole-body x-irradiation of guinea pigs on liver ribonucleic acid synthesis.
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pubmed:publicationType |
Journal Article
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