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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1970-9-6
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pubmed:abstractText |
Suspensions of cells of a marine pseudomonad washed with 0.05 m MgSO(4) showed an immediate increase in optical density (first-phase optical change) when the salt concentration of the suspending medium was increased; a subsequent slow decrease in optical density (second-phase optical change) occurred if K(+) was present. The rate of the second-phase change was similar to the rate of uptake of (42)K(+) by the cells. Glutamate increased the rate and extent of the second-phase change and produced a parallel increase in the rate and extent of uptake of (42)K(+). Citrate increased the extent of the second-phase change in cells adapted to oxidize citrate but not in unadapted cells. Adapted, but not unadapted, cells accumulated (14)C-citrate. The nonmetabolizable alpha-aminoisobutyric acid (AIB) also increased the extent of the second-phase change under conditions leading to the uptake of (14)C-AIB by the cells. Cells maintained in a salt solution optimal for the retention of intracellular solutes were found to contain 0.184 m K(+). In the same salt solution, cells preloaded with (42)K(+) retained the isotope, but they lost it rapidly when suspended in 0.05 m MgSO(4). The second-phase changes can be accounted for by the energy-dependent accumulation in an osmotically active form of K(+) and other metabolites by cells depleted of intracellular solutes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-13306891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-13306892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-13437225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-13549466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-13654736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-13909521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-13971086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-14021131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-14083907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-14129666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-14188701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-14249101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-14483070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-17840695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-4981061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-5344103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-5769176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-5940319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5429723-5941284
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aminoisobutyric Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Isotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Citrates,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamates,
http://linkedlifedata.com/resource/pubmed/chemical/Inulin,
http://linkedlifedata.com/resource/pubmed/chemical/Ions,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium Sulfate,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrogen,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
102
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
790-6
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:5429723-Aminoisobutyric Acids,
pubmed-meshheading:5429723-Biological Transport,
pubmed-meshheading:5429723-Carbon Isotopes,
pubmed-meshheading:5429723-Citrates,
pubmed-meshheading:5429723-Culture Media,
pubmed-meshheading:5429723-Glutamates,
pubmed-meshheading:5429723-Inulin,
pubmed-meshheading:5429723-Ions,
pubmed-meshheading:5429723-Magnesium Sulfate,
pubmed-meshheading:5429723-Nitrogen,
pubmed-meshheading:5429723-Osmosis,
pubmed-meshheading:5429723-Potassium,
pubmed-meshheading:5429723-Pseudomonas,
pubmed-meshheading:5429723-Sodium,
pubmed-meshheading:5429723-Spectrophotometry,
pubmed-meshheading:5429723-Water Microbiology
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pubmed:year |
1970
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pubmed:articleTitle |
Transport and retention of K+ and other metabolites in a marine pseudomonad and their relation to the mechanism of optical effects.
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pubmed:publicationType |
Journal Article
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