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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1980-6-25
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pubmed:abstractText |
While searching for true lysogens among oxytetracycline-producing Streptomyces rimosus strains, free phage particles were detected and isolated from a liquid culture of S. rimosus ATCC 10970 (R7). The actinophage, designated RP2, appears to be a typical temperate DNA phage producing turbid plaques on the sensitive strain S. rimosus R6. Electron microscopic examination of RP2 lysates showed that it belongs to group B of Bradley's morphological classification. The rate of RP2 adsoprption at 28 degrees C appeared to be low. The length of the latent period was about 6 h and the average burst size about 120 phage particles. The lysogenic nature of the host-virus system described was established on the basis of the following characteristics: spontaneous lysis frequency of 2 X 10(-6) per cell, resistance to curing with phage-specific antiserum, spontaneous curing frequency of less than 0.05% and immunity to superinfection with the homologous phage. Clear-plaque mutants of RP2, which failed to lysogenize sensitive cultures, arose at a frequency of 10(-5).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-1287
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
114
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
295-303
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pubmed:dateRevised |
2000-12-18
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pubmed:meshHeading |
pubmed-meshheading:541659-Adsorption,
pubmed-meshheading:541659-Bacteriophages,
pubmed-meshheading:541659-DNA, Viral,
pubmed-meshheading:541659-Lysogeny,
pubmed-meshheading:541659-Mutation,
pubmed-meshheading:541659-Oxytetracycline,
pubmed-meshheading:541659-Species Specificity,
pubmed-meshheading:541659-Streptomyces,
pubmed-meshheading:541659-Virus Replication
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pubmed:year |
1979
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pubmed:articleTitle |
Characterization and persistence of actinophage RP2 isolated from Streptomyces rimosus ATCC 10970.
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pubmed:publicationType |
Journal Article
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